Effect of Explant Origin and Medium Plant Growth Regulators on In Vitro Shoot Proliferation and Rooting of Salvia tomentosa, a Native Sage of the Northeastern Mediterranean Basin

Salvia tomentosa is a perennial sage of the maquis vegetation, strongly aromatic, with medicinal and ornamental value. Aiming to facilitate promotion species in floriculture sector, its micropropagation was studied terms effect explant origin medium plant growth regulators on vitro cultures establishment, shoot proliferation rooting. Shoot tip or single node explants excised from either grown seedlings greenhouse plants cuttings mature were cultured MS supplemented 6-benzyladenine (BA) at concentrations 0.0–3.2 mg L−1 combination 0.0, 0.01 0.1 1-naphthaleneacetic acid (NAA). Infections biggest problem for establishing plants, while showed hyperhydricity, especially nodal ones. In multiplication stage, high BA reduced response produce shoots as well length produced, although they increased number per responding explant, large hyperhydrated seedling origin. general, highest indexes, relevant normal shoots, given by hormone-free one low concentration, originated adult plants. The rooting rates (70–78%) achieved full half strength indole-3-butyric (IBA) medium. Increasing IBA resulted an increase root number, but decrease rate length. Ex acclimatization highly successful (92–96%) peat-perlite (1:1 v/v) substrate. protocol developed will contribute S. pharmaceutical industry be tool breeding programs targeting value products.